Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXO3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
Neural stem cells
treatment
LY294002
chip antibody
ChIP-grade anti-FOXO3 antibody “NFL"
phenotype
Huntington's disease
sirna
scrambled RNA
tissue
Neural stem cells
cell type
IPS cells differentiated into neural stem cells
passage number
7-12
genotype
72Q/19Q

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was immunoprecipitated using a NFL FOXO3 antibody (FOXO files) or mock antibody (IgG files). Whole cell extract files are labelled as WCE for each condition. Libraries were prepared by FASTERIS SA (Switzerland) using standard protocols from Illumina and sequenced with the Illumina HiSeq Genome Analyzer

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
3071473
Reads aligned (%)
49.2
Duplicates removed (%)
6.6
Number of peaks
36 (qval < 1E-05)

hg19

Number of total reads
3071473
Reads aligned (%)
48.8
Duplicates removed (%)
7.0
Number of peaks
53 (qval < 1E-05)

Base call quality data from DBCLS SRA